Pi
0.000358
MarkerSeek score
55.20
Primer available
yes
Label
ycf2
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf2_p1 | TTTCCAGGCGATCGGAAAAT | GTGGTGTAACGTGTATCCCC | 45.0 | 55.0 | 57.9 | 58.0 | 227 bp | 1.0 | 79.3 |
| ycf2_p2 | TTTCCAGGCGATCGGAAAAT | CGTGGTGTAACGTGTATCCC | 45.0 | 55.0 | 57.9 | 58.4 | 228 bp | 1.0 | 76.4 |
| ycf2_p3 | TTGTTCTTTCCAGGCGATCG | GTGGTGTAACGTGTATCCCC | 50.0 | 55.0 | 58.6 | 58.0 | 233 bp | 1.0 | 75.7 |
| ycf2_p4 | TTTCCAGGCGATCGGAAAAT | TCGTGGTGTAACGTGTATCC | 45.0 | 50.0 | 57.9 | 57.4 | 229 bp | 1.0 | 74.3 |
| ycf2_p5 | TTGTTCTTTCCAGGCGATCG | CGTGGTGTAACGTGTATCCC | 50.0 | 55.0 | 58.6 | 58.4 | 234 bp | 1.0 | 72.9 |
| ycf2_p1 | ATCGATAGGGCCTCATTGGT | GCTTCGACTCATATTCCCCA | 50.0 | 50.0 | 58.3 | 57.1 | 239 bp | 0.666667 | 57.6 |
| ycf2_p2 | ATCGATAGGGCCTCATTGGT | TGCTTCGACTCATATTCCCC | 50.0 | 50.0 | 58.3 | 57.1 | 240 bp | 0.666667 | 57.6 |
| ycf2_p3 | TCGATAGGGCCTCATTGGTA | GCTTCGACTCATATTCCCCA | 50.0 | 50.0 | 57.6 | 57.1 | 238 bp | 0.666667 | 56.6 |
| ycf2_p4 | TCGATAGGGCCTCATTGGTA | TGCTTCGACTCATATTCCCC | 50.0 | 50.0 | 57.6 | 57.1 | 239 bp | 0.666667 | 56.6 |
| ycf2_p5 | ATCGATAGGGCCTCATTGGT | GCTTCGACTCATATTCCCCAA | 50.0 | 47.6 | 58.3 | 57.8 | 239 bp | 0.666667 | 55.3 |