Hotspot

ycf1

SSC · 131173-136740 · 5568 bp

Back to Strombocarpa

Pi 0.00769

MarkerSeek score 49.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5568
variable_sites: 63
pi: 0.00769
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGATCGAACCCCAAAAATGC	GAGCGCTCCCAATTCTGTAA	50.0	50.0	58.1	58.0	535-536 bp	1.0	83.0
ycf1_p2	CCGATTCGCTATGGAACGAT	GAGCGCTCCCAATTCTGTAA	50.0	50.0	57.9	58.0	651-652 bp	1.0	82.7
ycf1_p3	CAAGATTTAGTCGACCCGCA	GAGCGCTCCCAATTCTGTAA	50.0	50.0	58.0	58.0	886-887 bp	1.0	82.3
ycf1_p4	GTCGACCCGCATATTCTGTT	GAGCGCTCCCAATTCTGTAA	50.0	50.0	58.1	58.0	877-878 bp	1.0	81.8
ycf1_p5	TCTGCTTCGATGTTGTTCGT	GAGCGCTCCCAATTCTGTAA	45.0	50.0	57.9	58.0	992-993 bp	1.0	81.0