Hotspot

clpP

LSC · 76205-78210 · 2006 bp

Back to Strombocarpa

Pi 0.00631

MarkerSeek score 40.40

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2006
variable_sites: 19
pi: 0.00631
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCCGGTAAGCGGTAAGAAGA	TTACGTTTCCGCATCAAAGC	50.0	45.0	58.2	57.4	2114-2126 bp	1.0	75.4
clpP_p2	TCCGGTAAGCGGTAAGAAGA	CCATTGTTACGTTTCCGCAT	50.0	45.0	58.2	57.1	2120-2132 bp	1.0	73.0
clpP_p3	TTGTGTATTTGCGCGGAATC	TTACGTTTCCGCATCAAAGC	45.0	45.0	57.5	57.4	2082-2094 bp	1.0	72.5
clpP_p4	CCGGTAAGCGGTAAGAAGAA	TTACGTTTCCGCATCAAAGC	50.0	45.0	57.3	57.4	2113-2125 bp	1.0	71.5
clpP_p5	TCCGGTAAGCGGTAAGAAGA	ACCCATTGTTACGTTTCCGC	50.0	50.0	58.2	59.1	2122-2134 bp	1.0	71.3