Hotspot

petA-psbJ

LSC · 68205-69523 · 1319 bp

Back to Strombocarpa

Pi 0.00455

MarkerSeek score 53.20

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1319
variable_sites: 9
pi: 0.00455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1354-1446 bp	1.0	80.5
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1364-1456 bp	1.0	79.7
petA-psbJ_p3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1348-1440 bp	1.0	79.6
petA-psbJ_p4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1349-1441 bp	1.0	79.6
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1369-1461 bp	1.0	79.1