Hotspot

ycf4-cemA

LSC · 65916-66334 · 419 bp

Back to Strombocarpa

Pi 0.00955

MarkerSeek score 52.40

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 6
pi: 0.00955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.1	58.1	513-528 bp	1.0	81.9
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	514-529 bp	1.0	81.8
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.1	58.1	512-527 bp	1.0	81.8
ycf4-cemA_p4	GAACAAAAAGCGGCGGAATT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.2	58.1	531-546 bp	1.0	80.8
ycf4-cemA_p5	GAACAAAAAGCGGCGGAATT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.2	58.1	530-545 bp	1.0	80.7