Hotspot

ndhC-trnV-UAC

LSC · 55747-56745 · 999 bp

Back to Strombocarpa

Pi 0.016
MarkerSeek score 60.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
999
variable_sites
24
pi
0.016
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCCCTTTATTAATCGGGG AAAAGATGCACGTTGGGTCT 50.0 45.0 57.3 58.0 976-1217 bp 1.0 80.2
ndhC-trnV-UAC_p2 TGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 905-1146 bp 1.0 78.9
ndhC-trnV-UAC_p3 TGGCCCTTTATTAATCGGGG GATGCACGTTGGGTCTGTAA 50.0 50.0 57.3 58.2 972-1213 bp 1.0 78.8
ndhC-trnV-UAC_p4 TGGCCCTTTATTAATCGGGG ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.3 57.7 971-1212 bp 1.0 78.2
ndhC-trnV-UAC_p5 TGGCCCTTTATTAATCGGGG ACGTTGGGTCTGTAAAGCAA 50.0 45.0 57.3 57.7 967-1208 bp 1.0 77.7