Hotspot

trnC-GCA-petN

LSC · 30543-31838 · 1296 bp

Back to Strombocarpa

Pi 0.00772

MarkerSeek score 62.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 15
pi: 0.00772
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1336-1362 bp	1.0	83.0
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1388-1414 bp	1.0	82.5
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.1	57.6	1397-1423 bp	1.0	81.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TCTTCCCCATACTACGAGGG	50.0	55.0	58.1	57.6	1387-1413 bp	1.0	81.2
trnC-GCA-petN_p5	GTATCATTTTGGCGGCATGG	AGAGTCCACTTCTTCCCCAT	50.0	50.0	57.9	57.6	1449-1475 bp	1.0	80.8