Hotspot

atpH-atpI

LSC · 15509-16914 · 1406 bp

Back to Strombocarpa

Pi 0.00427

MarkerSeek score 58.90

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1406
variable_sites: 9
pi: 0.00427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AATAGAAGCAAGCCCGACAG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.0	57.9	1518-1527 bp	1.0	81.2
atpH-atpI_p2	CAATAGAAGCAAGCCCGACA	TTTTGCAACTTTAGCTGCGG	50.0	45.0	58.3	57.9	1519-1528 bp	1.0	79.3
atpH-atpI_p3	CCTTCTACAGCTTGACCTGC	TTTTGCAACTTTAGCTGCGG	55.0	45.0	58.3	57.9	1565-1574 bp	1.0	79.2
atpH-atpI_p4	TCCCTTCTACAGCTTGACCT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.7	57.9	1567-1576 bp	1.0	78.7
atpH-atpI_p5	CCCTTCTACAGCTTGACCTG	TTTTGCAACTTTAGCTGCGG	55.0	45.0	57.6	57.9	1566-1575 bp	1.0	78.2