Hotspot

psbI-trnS-GCU

LSC · 8936-9207 · 272 bp

Back to Strombocarpa

Pi 0.0245

MarkerSeek score 52.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 272
variable_sites: 10
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	GTAATCCCGGACGTGAAGAA	TGGACTAAAGCGTCGGATTG	50.0	50.0	57.3	58.0	339-368 bp	1.0	80.9
psbI-trnS-GCU_p2	GTAATCCCGGACGTGAAGAA	GTGGACTAAAGCGTCGGATT	50.0	50.0	57.3	58.0	340-369 bp	1.0	80.9
psbI-trnS-GCU_p3	GTAATCCCGGACGTGAAGAA	TTTTTCGTACCGAGGGTTCG	50.0	50.0	57.3	58.2	302-331 bp	1.0	79.5
psbI-trnS-GCU_p4	GTAATCCCGGACGTGAAGAA	GACTAAAGCGTCGGATTGCT	50.0	50.0	57.3	58.4	337-366 bp	1.0	78.1
psbI-trnS-GCU_p5	GTAATCCCGGACGTGAAGAA	CTGAGTGGACTAAAGCGTCG	50.0	55.0	57.3	58.4	344-373 bp	1.0	77.9