Hotspot

trnQ-UUG-psbK

LSC · 7786-8462 · 677 bp

Back to Strombocarpa

Pi 0.0108

MarkerSeek score 52.80

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 11
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GATATGCTCTGGGACGGAAG	AGGCTTCAGGTAATTTCGCG	55.0	50.0	57.6	58.6	834-845 bp	1.0	75.4
trnQ-UUG-psbK_p2	GATATGCTCTGGGACGGAAG	GGCTTCAGGTAATTTCGCGA	55.0	50.0	57.6	58.6	833-844 bp	1.0	75.3
trnQ-UUG-psbK_p3	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTCGCG	50.0	50.0	58.7	58.6	792-803 bp	1.0	73.7
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTCGCGA	50.0	50.0	58.7	58.6	791-802 bp	1.0	73.7
trnQ-UUG-psbK_p5	AATGGATATGCTCTGGGACG	AGGCTTCAGGTAATTTCGCG	50.0	50.0	57.2	58.6	838-849 bp	1.0	72.2