Hotspot

rps16-trnQ-UUG

LSC · 6932-7713 · 782 bp

Back to Strombocarpa

Pi 0.0196

MarkerSeek score 56.00

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 782
variable_sites: 23
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	831-862 bp	1.0	85.5
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	833-864 bp	1.0	84.5
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	819-850 bp	1.0	84.0
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	834-865 bp	1.0	83.8
rps16-trnQ-UUG_p5	TGTCTTTCAAGTCGCACGTT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.4	58.1	832-863 bp	1.0	82.7