Hotspot

trnK-UUU-rps16

LSC · 4417-5760 · 1344 bp

Back to Strombocarpa

Pi 0.0179
MarkerSeek score 46.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1344
variable_sites
36
pi
0.0179
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCTGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1585-1624 bp 1.0 82.9
trnK-UUU-rps16_p2 CCTTTCTGGATCAGTCGTGG AAGCTAACGGATCGTGGAAC 55.0 50.0 58.0 58.0 2130-2168 bp 1.0 82.6
trnK-UUU-rps16_p3 CCTTTCTGGATCAGTCGTGG TGAGGTTGCGAATACGAGTG 55.0 50.0 58.0 58.0 1932-1971 bp 1.0 82.6
trnK-UUU-rps16_p4 CCTTTCTGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1723-1762 bp 1.0 82.5
trnK-UUU-rps16_p5 GGGCCAAGGATCGAAGATTT AAGCTAACGGATCGTGGAAC 50.0 50.0 57.9 58.0 2744-2788 bp 1.0 81.8