Hotspot

ycf2

LSC · 85887-91979 · 6093 bp

Back to Strobilanthes

Pi 0.013

MarkerSeek score 50.20

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 6093
variable_sites: 278
pi: 0.013
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	CCCAGGCGATCGGAAAATAA	ATGGAACGAGCCAATCATCC	50.0	50.0	58.0	58.0	1308-1317 bp	1.0	83.9
ycf2_p2	TCTTGAACCAGTCCAGGGAT	ATGTGGATCTCGGACCTAGG	50.0	55.0	58.0	58.0	736-745 bp	0.888889	79.8
ycf2_p3	TCTTGAACCAGTCCAGGGAT	ATCTCGGACCTAGGAATGGG	50.0	55.0	58.0	58.0	730-739 bp	0.888889	79.7
ycf2_p4	CCCAGGCGATCGGAAAATAA	ATGTGGATCTCGGACCTAGG	50.0	55.0	58.0	58.0	1097-1106 bp	0.888889	79.0
ycf2_p5	CCCAGGCGATCGGAAAATAA	ATCTCGGACCTAGGAATGGG	50.0	55.0	58.0	58.0	1091-1100 bp	0.888889	78.9