Hotspot

rpl32-trnL-UAG

SSC · 112382-112885 · 504 bp

Back to Strobilanthes

Pi 0.039
MarkerSeek score 78.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
504
variable_sites
54
pi
0.039
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGTTTTTGTGCGACAAACA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.2 57.9 497-576 bp 1.0 68.2
rpl32-trnL-UAG_p2 AGTTTTTGTGCGACAAACAA TCCTAAGAGCAGCGTGTCTA 35.0 50.0 55.2 57.9 496-575 bp 1.0 68.2
rpl32-trnL-UAG_p3 AAGTTTTTGTGCGACAAACA AGCAGCGTGTCTACCAATTT 35.0 45.0 55.2 57.8 490-569 bp 1.0 67.8
rpl32-trnL-UAG_p4 AGTTTTTGTGCGACAAACAA AGCAGCGTGTCTACCAATTT 35.0 45.0 55.2 57.8 489-568 bp 1.0 67.8
rpl32-trnL-UAG_p5 AGTTTTTGTGCGACAAACAA CTAAGAGCAGCGTGTCTACC 35.0 55.0 55.2 57.8 494-573 bp 1.0 67.7