Hotspot

ndhF-rpl32

SSC · 111639-112225 · 587 bp

Back to Strobilanthes

Pi 0.0296

MarkerSeek score 78.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 587
variable_sites: 60
pi: 0.0296
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TCCACCCATATTGAAATGCCT	TTAACGCTGCCCAATATCCC	42.9	50.0	57.3	58.0	648-792 bp	1.0	76.9
ndhF-rpl32_p2	CCACCCATATTGAAATGCCTG	TTAACGCTGCCCAATATCCC	47.6	50.0	57.3	58.0	647-791 bp	1.0	76.5
ndhF-rpl32_p3	TCCACCCATATTGAAATGCCT	GCTGCCCAATATCCCTTTCT	42.9	50.0	57.3	57.6	643-787 bp	1.0	74.2
ndhF-rpl32_p4	CCACCCATATTGAAATGCCTG	GCTGCCCAATATCCCTTTCT	47.6	50.0	57.3	57.6	642-786 bp	1.0	73.8
ndhF-rpl32_p5	TGAAAGGTATCATCCACCCA	TTAACGCTGCCCAATATCCC	45.0	50.0	55.8	58.0	660-804 bp	1.0	72.8