Hotspot

psaJ-rpl33

LSC · 66462-66802 · 341 bp

Back to Strobilanthes

Pi 0.0355

MarkerSeek score 73.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 32
pi: 0.0355
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGCTGACATTCCCCTTTTT	GCATCTTTCCTCTTAGCCAT	50.0	45.0	59.4	55.2	369-402 bp	1.0	57.0
psaJ-rpl33_p2	GCGCTGACATTCCCCTTTT	GCATCTTTCCTCTTAGCCAT	52.6	45.0	58.7	55.2	369-402 bp	1.0	54.3
psaJ-rpl33_p3	GCGCTGACATTCCCCTTT	GCATCTTTCCTCTTAGCCAT	55.6	45.0	58.0	55.2	369-402 bp	1.0	52.2
psaJ-rpl33_p4	TGCGCTGACATTCCCCTTTT	GCATCTTTCCTCTTAGCCAT	50.0	45.0	60.5	55.2	370-403 bp	1.0	50.7
psaJ-rpl33_p5	ATGCGCTGACATTCCCCTTT	GCATCTTTCCTCTTAGCCAT	50.0	45.0	60.3	55.2	371-404 bp	1.0	50.6