Hotspot

accD-psaI

LSC · 58437-59187 · 751 bp

Back to Strobilanthes

Pi 0.0157

MarkerSeek score 67.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 37
pi: 0.0157
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CTGCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	59.0	58.0	846-899 bp	1.0	78.8
accD-psaI_p2	GCACGCCTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	59.0	58.0	844-897 bp	1.0	78.8
accD-psaI_p3	CTGCACGCCTTCTTTCCTTT	TTGCCGGAAATACTAAGCCC	50.0	50.0	59.0	58.0	837-890 bp	1.0	78.7
accD-psaI_p4	GCACGCCTTCTTTCCTTTGA	TTGCCGGAAATACTAAGCCC	50.0	50.0	59.0	58.0	835-888 bp	1.0	78.7
accD-psaI_p5	TGCACGCCTTCTTTCCTTT	CCATTGCAATTGCCGGAAAT	47.4	45.0	57.9	58.0	845-898 bp	1.0	78.3