Hotspot

rps4-trnT-UGU

LSC · 45505-46026 · 522 bp

Back to Strobilanthes

Pi 0.0338

MarkerSeek score 82.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 54
pi: 0.0338
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGTCCTGGTAAAGTCCCCAG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	647-719 bp	1.0	86.5
rps4-trnT-UGU_p2	AGTCCTGGTAAAGTCCCCAG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	615-687 bp	1.0	85.9
rps4-trnT-UGU_p3	GCTTCCAGCTTTAGTCCCTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.5	57.9	675-747 bp	1.0	85.0
rps4-trnT-UGU_p4	GCTTCCAGCTTTAGTCCCTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.5	57.8	643-715 bp	1.0	84.4
rps4-trnT-UGU_p5	AGTCCTGGTAAAGTCCCCAG	ATGTAGGCCTGCTTAGCTCA	55.0	50.0	58.3	58.5	663-735 bp	1.0	83.4