Hotspot

trnG-GCC-trnM-CAU

LSC · 35905-36089 · 185 bp

Back to Strobilanthes

Pi 0.0288

MarkerSeek score 67.90

Primer available yes

Label trnG-GCC-trnM-CAU

Feature Metadata

label_name: trnG-GCC-trnM-CAU
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 17
pi: 0.0288
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-trnM-CAU_p1	AGAAGATGCGGGTTCGATTC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	251-255 bp	1.0	84.6
trnG-GCC-trnM-CAU_p2	GAGAAGATGCGGGTTCGATT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.1	58.0	252-256 bp	1.0	84.6
trnG-GCC-trnM-CAU_p3	AGAAGATGCGGGTTCGATTC	GTCACGGGTTCAAATCCTGT	50.0	50.0	58.1	58.1	245-249 bp	1.0	84.2
trnG-GCC-trnM-CAU_p4	AGAAGATGCGGGTTCGATTC	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	287-291 bp	1.0	84.0
trnG-GCC-trnM-CAU_p5	GAGAAGATGCGGGTTCGATT	GGGTAGAGCAGTTTGGTAGC	50.0	55.0	58.1	58.0	288-292 bp	1.0	84.0