Hotspot

atpF-atpH

LSC · 12013-12407 · 395 bp

Back to Strobilanthes

Pi 0.0305

MarkerSeek score 71.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 33
pi: 0.0305
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	AGAAAGAGTACCATGCTGCG	TGATTTCTGCTGCTTCCGTT	50.0	45.0	58.0	58.1	987-1024 bp	1.0	86.5
atpF-atpH_p2	ACAGGTTGTCAATTCAGCGT	TGATTTCTGCTGCTTCCGTT	45.0	45.0	58.0	58.1	1132-1189 bp	1.0	86.1
atpF-atpH_p3	AGAAAGAGTACCATGCTGCG	CGGAACCAAGAAACGGAAGA	50.0	50.0	58.0	58.1	1262-1292 bp	1.0	85.9
atpF-atpH_p4	AGAAAGAGTACCATGCTGCG	TCGGAACCAAGAAACGGAAG	50.0	50.0	58.0	58.1	1263-1293 bp	1.0	85.9
atpF-atpH_p5	AGAAAGAGTACCATGCTGCG	AACCCTCGTATTCTTGTGGC	50.0	50.0	58.0	57.9	1343-1372 bp	0.888889	80.8