Hotspot

trnK-UUU-rps16

LSC · 3057-3471 · 415 bp

Back to Strobilanthes

Pi 0.0282

MarkerSeek score 68.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 34
pi: 0.0282
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AACCCAATCGCCCTTTTGAT	TGCTGCCATTTTGAAGGGAT	45.0	45.0	58.1	58.1	1567-1655 bp	1.0	87.3
trnK-UUU-rps16_p2	ACCCAATCGCCCTTTTGATT	TGCTGCCATTTTGAAGGGAT	45.0	45.0	58.1	58.1	1566-1654 bp	1.0	87.3
trnK-UUU-rps16_p3	ATAGAATCCCCTCGTCCTGG	TGCTGCCATTTTGAAGGGAT	55.0	45.0	58.0	58.1	1515 bp	0.111111	43.8
trnK-UUU-rps16_p4	ATAGAATCCCCTCGTCCTGG	ATCAACAGGCCATGGGATTC	55.0	50.0	58.0	57.9	1566 bp	0.111111	43.7
trnK-UUU-rps16_p5	ATAGAATCCCCTCGTCCTGG	GAATCAACAGGCCATGGGAT	55.0	50.0	58.0	57.9	1568 bp	0.111111	43.7