Hotspot

ycf1

SSC · 123201-128552 · 5352 bp

Back to Strigosella

Pi 0.0134

MarkerSeek score 49.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5352
variable_sites: 136
pi: 0.0134
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTTTACGCCTTTGGGAACG	ACATGCCGAAGTGATGGAAA	50.0	45.0	58.0	57.8	914-938 bp	1.0	81.1
ycf1_p2	GTTTTACGCCTTTGGGAACG	AAAAGCACCCTCTGACCAAG	50.0	50.0	58.0	58.0	810-834 bp	0.5	62.0
ycf1_p3	GTTTTACGCCTTTGGGAACG	GAAAAAGCACCCTCTGACCA	50.0	50.0	58.0	58.0	812-836 bp	0.5	62.0
ycf1_p4	GGACCACTCGGGAAATTGAA	AAAAGCACCCTCTGACCAAG	50.0	50.0	57.8	58.0	1168 bp	0.25	48.3
ycf1_p5	GGACCACTCGGGAAATTGAA	GAAAAAGCACCCTCTGACCA	50.0	50.0	57.8	58.0	1170 bp	0.25	48.3