Hotspot

petD

LSC · 75560-76752 · 1193 bp

Back to Strigosella

Pi 0.00699

MarkerSeek score 61.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1193
variable_sites: 16
pi: 0.00699
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TCCTTCAACTCCACAGTATTGT	ACTCCCTAGATACCCACGTC	40.9	55.0	57.0	57.6	1318-1323 bp	1.0	54.3
petD_p2	TCCTTCAACTCCACAGTATTGT	CTCCCTAGATACCCACGTCT	40.9	55.0	57.0	57.3	1317-1322 bp	1.0	51.9
petD_p3	TTTTCCTTCAACTCCACAGT	ACTCCCTAGATACCCACGTC	40.0	55.0	54.7	57.6	1321-1326 bp	1.0	51.7
petD_p4	TTCCTTCAACTCCACAGTATTGT	ACTCCCTAGATACCCACGTC	39.1	55.0	57.7	57.6	1319-1324 bp	1.0	51.7
petD_p5	TTTTTCCTTCAACTCCACAGT	ACTCCCTAGATACCCACGTC	38.1	55.0	55.5	57.6	1322-1327 bp	1.0	50.5