Hotspot

rpl32-trnL-CUA

SSC · 112817-113604 · 788 bp

Back to Strigosella

Pi 0.019

MarkerSeek score 57.70

Primer available yes

Label rpl32-trnL-CUA

Feature Metadata

label_name: rpl32-trnL-CUA
feature_type: igs
region: SSC
length_bp: 788
variable_sites: 29
pi: 0.019
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-CUA_p1	GCACTCTCGTACGGATTTCA	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.7	58.0	1757-1762 bp	1.0	80.1
rpl32-trnL-CUA_p2	AAATGAGCACTCTCGTACGG	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.7	58.0	1763-1768 bp	1.0	80.0
rpl32-trnL-CUA_p3	GCACTCTCGTACGGATTTCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	1216-1221 bp	1.0	80.0
rpl32-trnL-CUA_p4	AAATGAGCACTCTCGTACGG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.7	57.9	1222-1227 bp	1.0	80.0
rpl32-trnL-CUA_p5	ATGAGCACTCTCGTACGGAT	TGCAGACTGAGGCATTTTGT	50.0	45.0	58.3	58.0	1761-1766 bp	1.0	79.7