Hotspot

rps18-rpl20

LSC · 67216-67487 · 272 bp

Back to Strigosella

Pi 0.0141

MarkerSeek score 62.40

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 272
variable_sites: 7
pi: 0.0141
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	GAAAGAAGTGAGTCGACCCC	AACCGGAAAATACTTGCACA	55.0	40.0	57.9	55.9	404-407 bp	1.0	63.8
rps18-rpl20_p2	GAAAGAAGTGAGTCGACCCC	ACCGGAAAATACTTGCACAA	55.0	40.0	57.9	55.9	403-406 bp	1.0	63.8
rps18-rpl20_p3	GAAGTGAGTCGACCCCTAGA	AACCGGAAAATACTTGCACA	55.0	40.0	57.9	55.9	400-403 bp	1.0	63.5
rps18-rpl20_p4	GAAGTGAGTCGACCCCTAGA	ACCGGAAAATACTTGCACAA	55.0	40.0	57.9	55.9	399-402 bp	1.0	63.5
rps18-rpl20_p5	AGAAGTGAGTCGACCCCTAG	ACCGGAAAATACTTGCACAA	55.0	40.0	57.9	55.9	400-403 bp	1.0	63.4