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petA-psbJ

LSC · 61676-62585 · 910 bp

Back to Strigosella

Pi 0.0132

MarkerSeek score 70.30

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 910
variable_sites: 24
pi: 0.0132
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ACGTGTCCAAGGACTTTTGT	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.6	58.1	1097-1152 bp	1.0	70.9
petA-psbJ_p2	ACGTGTCCAAGGACTTTTGT	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.6	57.8	1098-1153 bp	1.0	70.2
petA-psbJ_p3	TAGGATCGGTTGTTTTGGCA	GCTGGTATTCTTGTGATCGGT	45.0	47.6	57.4	58.1	1071-1126 bp	1.0	69.8
petA-psbJ_p4	TAGGATCGGTTGTTTTGGCA	AGCTGGTATTCTTGTGATCGG	45.0	47.6	57.4	57.8	1072-1127 bp	1.0	69.0
petA-psbJ_p5	TCCATTACGTGTCCAAGGAC	GCTGGTATTCTTGTGATCGGT	50.0	47.6	57.2	58.1	1103-1158 bp	1.0	68.2