Hotspot

ndhK-ndhC

LSC · 49030-49096 · 67 bp

Back to Strigosella

Pi 0.00758

MarkerSeek score 62.60

Primer available yes

Label ndhK-ndhC

Feature Metadata

label_name: ndhK-ndhC
feature_type: igs
region: LSC
length_bp: 67
variable_sites: 1
pi: 0.00758
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK-ndhC_p1	GGCTGGAAAGTCTTGACCAA	ATGGCGAAAGGGAGCATTAG	50.0	50.0	58.0	58.0	192-198 bp	1.0	82.5
ndhK-ndhC_p2	GGCTGGAAAGTCTTGACCAA	GGCGAAAGGGAGCATTAGAA	50.0	50.0	58.0	58.0	190-196 bp	1.0	82.4
ndhK-ndhC_p3	GGCTGGAAAGTCTTGACCAA	TTTATGCATGGCGAAAGGGA	50.0	45.0	58.0	57.9	199-205 bp	1.0	81.5
ndhK-ndhC_p4	GGCTGGAAAGTCTTGACCAA	GTTTATGCATGGCGAAAGGG	50.0	50.0	58.0	57.8	200-206 bp	1.0	80.9
ndhK-ndhC_p5	GGCTGGAAAGTCTTGACCAA	TGGGCAATGAGTTTCGATGT	50.0	45.0	58.0	57.8	281-287 bp	1.0	80.2