Hotspot

trnT-ACC-psbD

LSC · 30439-31703 · 1265 bp

Back to Strigosella

Pi 0.0121

MarkerSeek score 60.10

Primer available yes

Label trnT-ACC-psbD

Feature Metadata

label_name: trnT-ACC-psbD
feature_type: igs
region: LSC
length_bp: 1265
variable_sites: 27
pi: 0.0121
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-ACC-psbD_p1	GTGGTAGAGTAACGCCATGG	CAAAAACAAAGCGGTCCCTC	55.0	50.0	58.1	57.9	1405-1432 bp	1.0	82.0
trnT-ACC-psbD_p2	TCAGTGGTAGAGTAACGCCA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.1	57.9	1408-1435 bp	1.0	81.8
trnT-ACC-psbD_p3	GGCGTAAGTCATCGGTTCAA	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.3	57.9	1382-1409 bp	1.0	80.3
trnT-ACC-psbD_p4	TGGTAGAGTAACGCCATGGT	CAAAAACAAAGCGGTCCCTC	50.0	50.0	58.4	57.9	1404-1431 bp	1.0	79.0
trnT-ACC-psbD_p5	GTGGTAGAGTAACGCCATGG	TCCCTCCGTAACCAGTCATC	55.0	55.0	58.1	58.5	1391-1418 bp	1.0	78.9