Hotspot

psbM-trnD-GAC

LSC · 27799-28826 · 1028 bp

Back to Strigosella

Pi 0.0112

MarkerSeek score 61.80

Primer available yes

Label psbM-trnD-GAC

Feature Metadata

label_name: psbM-trnD-GAC
feature_type: igs
region: LSC
length_bp: 1028
variable_sites: 20
pi: 0.0112
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GAC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1139-1152 bp	1.0	55.2
psbM-trnD-GAC_p2	AGAATGAAGAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.2	57.0	1140-1153 bp	1.0	51.1
psbM-trnD-GAC_p3	AGAATGAAGAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	45.0	52.6	55.2	57.8	1138-1151 bp	1.0	49.2
psbM-trnD-GAC_p4	AGAATGAAGAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	45.0	47.6	55.2	58.5	1141-1154 bp	1.0	47.1
psbM-trnD-GAC_p5	TAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	42.9	50.0	55.2	58.5	1140-1153 bp	1.0	47.0