Hotspot

ndhB-minD

Genome · 113750-115622 · 1873 bp

Back to Streptosarcina

Pi 0.194

MarkerSeek score 82.30

Primer available yes

Label ndhB-minD

Feature Metadata

label_name: ndhB-minD
feature_type: igs
region: Genome
length_bp: 1873
variable_sites: 358
pi: 0.194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-minD_p1	CACTCTCGTTCGTAGTCTGC	ATCTTCTCTCTGTCCACGGT	55.0	50.0	58.1	57.8	1939 bp	0.5	57.3
ndhB-minD_p2	TTGGTGTGAGCCCATTCATC	ATCTTCTCTCTGTCCACGGT	50.0	50.0	58.2	57.8	1973 bp	0.5	56.9
ndhB-minD_p3	AGGACACTCTCGTTCGTAGT	ATCTTCTCTCTGTCCACGGT	50.0	50.0	57.8	57.8	1943 bp	0.5	56.8
ndhB-minD_p4	GTAGTCTGCCTCTCTCGTGA	ATCTTCTCTCTGTCCACGGT	55.0	50.0	58.3	57.8	1928 bp	0.5	56.1
ndhB-minD_p5	ATTCATCTCCCTAGCCCAGG	ATCTTCTCTCTGTCCACGGT	55.0	50.0	58.3	57.8	1960 bp	0.5	56.1