Hotspot

orf340-trnI(gau)

Genome · 99459-100289 · 831 bp

Back to Streptosarcina

Pi 0.327

MarkerSeek score 80.50

Primer available yes

Label orf340-trnI(gau)

Feature Metadata

label_name: orf340-trnI(gau)
feature_type: igs
region: Genome
length_bp: 831
variable_sites: 267
pi: 0.327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf340-trnI(gau)_p1	GCAATTGAGAGCGAAGGAGA	TTGAAGCCTACGTCATGCTC	50.0	50.0	58.0	58.0	1842 bp	0.5	59.8
orf340-trnI(gau)_p2	CTCAGCTTCGTAGTCGCTTT	TTGAAGCCTACGTCATGCTC	50.0	50.0	58.0	58.0	1523 bp	0.5	59.8
orf340-trnI(gau)_p3	CGAGATGCTGTTCGTGAAGA	TTGAAGCCTACGTCATGCTC	50.0	50.0	58.0	58.0	1749 bp	0.5	59.8
orf340-trnI(gau)_p4	GCAATTGAGAGCGAAGGAGA	AAGCCTACGTCATGCTCTTG	50.0	50.0	58.0	58.0	1839 bp	0.5	59.7
orf340-trnI(gau)_p5	GCAATTGAGAGCGAAGGAGA	CTTGAAGCCTACGTCATGCT	50.0	50.0	58.0	58.0	1843 bp	0.5	59.7