Hotspot

trnV(uac)-trnMe(cau)

Genome · 40783-41458 · 676 bp

Back to Streptosarcina

Pi 0.0536

MarkerSeek score 80.00

Primer available yes

Label trnV(uac)-trnMe(cau)

Feature Metadata

label_name: trnV(uac)-trnMe(cau)
feature_type: igs
region: Genome
length_bp: 676
variable_sites: 36
pi: 0.0536
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV(uac)-trnMe(cau)_p1	GAGCTAGAAGGCCACAGAAC	TGAACCAATGACTTTCGCCT	55.0	45.0	58.0	57.7	831-843 bp	1.0	81.5
trnV(uac)-trnMe(cau)_p2	GGGCTATACGGATTCGAACC	TGAACCAATGACTTTCGCCT	55.0	45.0	57.9	57.7	803-815 bp	1.0	81.2
trnV(uac)-trnMe(cau)_p3	ATGAGCTAGAAGGCCACAGA	TGAACCAATGACTTTCGCCT	50.0	45.0	58.1	57.7	833-845 bp	1.0	80.6
trnV(uac)-trnMe(cau)_p4	CGAACCGTAGACAATCTCGG	TGAACCAATGACTTTCGCCT	55.0	45.0	58.2	57.7	789-801 bp	1.0	80.5
trnV(uac)-trnMe(cau)_p5	AAGGCCACAGAACAAGGTTT	TGAACCAATGACTTTCGCCT	45.0	45.0	57.8	57.7	824-836 bp	1.0	80.4