Hotspot

chlI-psbC

Genome · 13605-15572 · 1968 bp

Back to Streptosarcina

Pi 0.147

MarkerSeek score 85.00

Primer available yes

Label chlI-psbC

Feature Metadata

label_name: chlI-psbC
feature_type: igs
region: Genome
length_bp: 1968
variable_sites: 279
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 85.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlI-psbC_p1	GGTGCAAGAGAAGTTTAGCC	GCAGGTTTTGAGAAGGGGAT	50.0	50.0	56.7	57.8	2080 bp	0.5	48.0
chlI-psbC_p2	GGTGCAAGAGAAGTTTAGCCA	GCAGGTTTTGAGAAGGGGAT	47.6	50.0	58.5	57.8	2080 bp	0.5	46.3
chlI-psbC_p3	TGGTGCAAGAGAAGTTTAGCC	GCAGGTTTTGAGAAGGGGAT	47.6	50.0	58.5	57.8	2081 bp	0.5	46.3
chlI-psbC_p4	GGTGCAAGAGAAGTTTAGCC	GACACCGAACCCGTACTTTC	50.0	55.0	56.7	58.6	2053 bp	0.5	45.1
chlI-psbC_p5	GGTGCAAGAGAAGTTTAGCC	AACCCGTACTTTCTATGGGC	50.0	50.0	56.7	57.3	2046 bp	0.5	44.1