Hotspot

ycf12-trnL(uaa)

Genome · 6264-8209 · 1946 bp

Back to Streptosarcina

Pi 0.197

MarkerSeek score 97.10

Primer available yes

Label ycf12-trnL(uaa)

Feature Metadata

label_name: ycf12-trnL(uaa)
feature_type: igs
region: Genome
length_bp: 1946
variable_sites: 356
pi: 0.197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 97.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf12-trnL(uaa)_p1	GTTCTGTCAGGTCCCCTAGT	TTGAGACATAGGTTCGCACG	55.0	50.0	58.1	58.0	2092 bp	0.5	59.4
ycf12-trnL(uaa)_p2	TTCTGTCAGGTCCCCTAGTG	TTGAGACATAGGTTCGCACG	55.0	50.0	58.1	58.0	2091 bp	0.5	59.4
ycf12-trnL(uaa)_p3	TGCACTCATCGTTCTGTCAG	TTGAGACATAGGTTCGCACG	50.0	50.0	57.9	58.0	2102 bp	0.5	59.4
ycf12-trnL(uaa)_p4	AGCTAGCTGTTCTTGCACTC	TTGAGACATAGGTTCGCACG	50.0	50.0	57.9	58.0	2115 bp	0.5	59.2
ycf12-trnL(uaa)_p5	CTAGTGATAGCCCTGCTTGC	TTGAGACATAGGTTCGCACG	55.0	50.0	58.1	58.0	2077 bp	0.5	58.9