Hotspot

orf490-accD

Genome · 1575-3518 · 1944 bp

Back to Streptosarcina

Pi 0.306

MarkerSeek score 82.70

Primer available yes

Label orf490-accD

Feature Metadata

label_name: orf490-accD
feature_type: igs
region: Genome
length_bp: 1944
variable_sites: 567
pi: 0.306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
orf490-accD_p1	CATACCGGTCAGTCACAAGG	CCATGCTGCCTCCCATAAAT	55.0	50.0	58.0	58.0	2900 bp	0.5	59.9
orf490-accD_p2	CACCTGATCGATGAACTGCA	CCATGCTGCCTCCCATAAAT	50.0	50.0	58.0	58.0	2701 bp	0.5	59.8
orf490-accD_p3	TGCACCTGATCGATGAACTG	CCATGCTGCCTCCCATAAAT	50.0	50.0	58.0	58.0	2703 bp	0.5	59.8
orf490-accD_p4	ACCTGATCGATGAACTGCAC	CCATGCTGCCTCCCATAAAT	50.0	50.0	58.0	58.0	2700 bp	0.5	59.8
orf490-accD_p5	AGCCAGACTCAACCAGTAGT	CCATGCTGCCTCCCATAAAT	50.0	50.0	58.0	58.0	2419 bp	0.5	59.8