Hotspot

trnC-GCA

LSC · 28830-28900 · 71 bp

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Pi 0.0141

MarkerSeek score 60.60

Primer available yes

Label trnC-GCA

Feature Metadata

label_name: trnC-GCA
feature_type: tRNA
region: LSC
length_bp: 71
variable_sites: 1
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA_p1	GCAAGTTCAAATTTGCGACAG	TCAACGGCACAGAGTCAAAA	42.9	45.0	57.2	58.0	1360-1362 bp	1.0	65.9
trnC-GCA_p2	GCAAGTTCAAATTTGCGACAG	CCTCTATAGGCCACTCCTCC	42.9	60.0	57.2	58.1	1523-1525 bp	1.0	65.6
trnC-GCA_p3	GCAAGTTCAAATTTGCGACAG	CTCTATAGGCCACTCCTCCC	42.9	60.0	57.2	58.1	1522-1524 bp	1.0	65.6
trnC-GCA_p4	GCAAGTTCAAATTTGCGACAG	CTCCTCCCCCATACTACGAG	42.9	60.0	57.2	58.1	1510-1512 bp	1.0	65.4
trnC-GCA_p5	AGTTCAAATTTGCGACAGGT	TCAACGGCACAGAGTCAAAA	40.0	45.0	56.2	58.0	1357-1359 bp	1.0	65.3