Hotspot

atpB

LSC · 54355-55845 · 1491 bp

Back to Streptopus

Pi 0.00805

MarkerSeek score 55.30

Primer available yes

Label atpB

Feature Metadata

label_name: atpB
feature_type: gene
region: LSC
length_bp: 1491
variable_sites: 12
pi: 0.00805
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB_p1	TCGATTAGGGGTCAGTACACA	ACTGATCAATTTGCTATCGGACA	47.6	39.1	57.9	58.2	1601-1607 bp	1.0	46.0
atpB_p2	TCGATTAGGGGTCAGTACACA	CTGATCAATTTGCTATCGGACA	47.6	40.9	57.9	56.6	1600-1606 bp	1.0	44.6
atpB_p3	TCGATTAGGGGTCAGTACACA	ACTGATCAATTTGCTATCGGAC	47.6	40.9	57.9	56.6	1601-1607 bp	1.0	44.6
atpB_p4	TCGATTAGGGGTCAGTACACA	TGATCAATTTGCTATCGGACA	47.6	38.1	57.9	55.4	1599-1605 bp	1.0	43.0
atpB_p5	TCGATTAGGGGTCAGTACACA	AACTGATCAATTTGCTATCGGAC	47.6	39.1	57.9	57.3	1602-1608 bp	1.0	41.9