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rps8-rpl14

LSC · 82941-83192 · 252 bp

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Pi 0.0516

MarkerSeek score 81.40

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 13
pi: 0.0516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.2	58.0	419-420 bp	1.0	76.3
rps8-rpl14_p2	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	45.0	45.0	57.2	58.1	417-418 bp	1.0	75.5
rps8-rpl14_p3	ATGCTATTCGAACCGTTCCT	GAACTCGAGTTTTTGGTGCG	45.0	50.0	57.0	58.0	420-421 bp	1.0	74.5
rps8-rpl14_p4	TGCTATTCGAACCGTTCCTTT	GAACTCGAGTTTTTGGTGCG	42.9	50.0	58.0	58.0	419-420 bp	1.0	73.9
rps8-rpl14_p5	TGCTATTCGAACCGTTCCTT	AACTCGAGTTTTTGGTGCGA	45.0	45.0	57.2	58.3	418-419 bp	1.0	73.7