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accD-psaI

LSC · 60249-61141 · 893 bp

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Pi 0.00342

MarkerSeek score 62.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 893
variable_sites: 3
pi: 0.00342
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	993-1003 bp	1.0	80.3
accD-psaI_p2	TCAGCTCCACGGTTTCTTTC	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.1	57.9	999-1009 bp	1.0	79.6
accD-psaI_p3	TTCAGCTCCACGGTTTCTTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	994-1004 bp	1.0	78.5
accD-psaI_p4	TTTCAGCTCCACGGTTTCTT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.7	58.0	995-1005 bp	1.0	78.5
accD-psaI_p5	TTCAGCTCCACGGTTTCTTT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.7	57.9	1000-1010 bp	1.0	77.9