Hotspot

trnT-UGU-trnL-UAA

LSC · 47145-48213 · 1069 bp

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Pi 0.00474
MarkerSeek score 60.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
1069
variable_sites
5
pi
0.00474
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 1130-1274 bp 1.0 78.2
trnT-UGU-trnL-UAA_p2 GGAATCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 1162-1306 bp 1.0 77.3
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CGTCTACCGATTTCGCCATA 55.0 50.0 57.9 57.6 1124-1268 bp 1.0 76.9
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GGTTTCCATACCAAGGCTCA 55.0 50.0 57.9 57.5 1162-1306 bp 1.0 76.1
trnT-UGU-trnL-UAA_p5 GCGATGCTCTAACCTCTGAG CAGGTTTCCATACCAAGGCT 55.0 50.0 57.9 57.5 1164-1308 bp 1.0 76.1