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psaA-ycf3

LSC · 42379-43055 · 677 bp

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Pi 0.00153

MarkerSeek score 62.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 677
variable_sites: 1
pi: 0.00153
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GTCTGGCCCATTCCTCAAAA	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	795 bp	1.0	80.4
psaA-ycf3_p2	TGGCCCATTCCTCAAAAGAC	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.1	57.8	792 bp	1.0	80.4
psaA-ycf3_p3	TGTTCTCGAGAAATGGCCTG	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	815 bp	1.0	80.2
psaA-ycf3_p4	GTCTGGCCCATTCCTCAAAA	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	799 bp	1.0	80.2
psaA-ycf3_p5	TGGCCCATTCCTCAAAAGAC	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.1	58.2	796 bp	1.0	80.2