Hotspot

trnS-GCU-trnG-UCC

LSC · 8755-10050 · 1296 bp

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Pi 0.076

MarkerSeek score 65.60

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1296
variable_sites: 38
pi: 0.076
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.39
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	575-1370 bp	1.0	58.5
trnS-GCU-trnG-UCC_p2	TGATGAGGGATAGCGGAGAG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	638-1433 bp	1.0	58.0
trnS-GCU-trnG-UCC_p3	AAGTGATGAGGGATAGCGGA	ACGAATCACACTTTTACCACT	50.0	38.1	57.9	55.5	641-1436 bp	1.0	57.9
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	577-1372 bp	1.0	57.5
trnS-GCU-trnG-UCC_p5	CAAGTGATGAGGGATAGCGG	ACGAATCACACTTTTACCACT	55.0	38.1	57.8	55.5	642-1437 bp	1.0	57.4