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trnK-UUU-rps16

LSC · 4082-4805 · 724 bp

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Pi 0.00277

MarkerSeek score 60.60

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 724
variable_sites: 2
pi: 0.00277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GCTCAACCCACAGTAACTGT	50.0	50.0	58.2	57.7	828-830 bp	1.0	77.0
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GCTCAACCCACAGTAACTGT	50.0	50.0	58.2	57.7	827-829 bp	1.0	77.0
trnK-UUU-rps16_p3	AAAGCCGAGTACTCTACCGT	CTTGAAAATGGCGCTCAACC	50.0	50.0	58.2	58.3	840-842 bp	1.0	76.6
trnK-UUU-rps16_p4	AAGCCGAGTACTCTACCGTT	CCTTGAAAATGGCGCTCAAC	50.0	50.0	58.2	58.3	840-842 bp	1.0	76.6
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	CTTGAAAATGGCGCTCAACC	50.0	50.0	58.2	58.3	839-841 bp	1.0	76.6