primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GCTCAACCCACAGTAACTGT	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.424537	4082	4805	828-830	1.000000	77.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GCTCAACCCACAGTAACTGT	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.424537	4082	4805	827-829	1.000000	77.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	CTTGAAAATGGCGCTCAACC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	4082	4805	840-842	1.000000	76.6
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	CCTTGAAAATGGCGCTCAAC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	4082	4805	840-842	1.000000	76.6
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	CTTGAAAATGGCGCTCAACC	20	20	50.0	50.0	58.2	58.3	20.3	7.6	0.466716	4082	4805	839-841	1.000000	76.6
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	20	21	45.0	38.1	58.0	55.5	0.0	0.0	3.574049	8755	10050	575-1370	1.000000	58.5
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	TGATGAGGGATAGCGGAGAG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	58.1	55.5	0.0	0.0	3.619337	8755	10050	638-1433	1.000000	58.0
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	AAGTGATGAGGGATAGCGGA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	57.9	55.5	0.0	0.0	3.623947	8755	10050	641-1436	1.000000	57.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	20	23	45.0	39.1	58.0	57.3	0.0	0.0	3.706765	8755	10050	577-1372	1.000000	57.5
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	CAAGTGATGAGGGATAGCGG	ACGAATCACACTTTTACCACT	20	21	55.0	38.1	57.8	55.5	0.0	0.0	3.696665	8755	10050	642-1437	1.000000	57.4
trnC-GCA_p1	trnC-GCA	1	GCAAGTTCAAATTTGCGACAG	TCAACGGCACAGAGTCAAAA	21	20	42.9	45.0	57.2	58.0	13.5	0.0	1.789779	28830	28900	1360-1362	1.000000	65.9
trnC-GCA_p2	trnC-GCA	2	GCAAGTTCAAATTTGCGACAG	CCTCTATAGGCCACTCCTCC	21	20	42.9	60.0	57.2	58.1	13.5	5.0	1.826886	28830	28900	1523-1525	1.000000	65.6
trnC-GCA_p3	trnC-GCA	3	GCAAGTTCAAATTTGCGACAG	CTCTATAGGCCACTCCTCCC	21	20	42.9	60.0	57.2	58.1	13.5	5.0	1.826886	28830	28900	1522-1524	1.000000	65.6
trnC-GCA_p4	trnC-GCA	4	GCAAGTTCAAATTTGCGACAG	CTCCTCCCCCATACTACGAG	21	20	42.9	60.0	57.2	58.1	13.5	0.0	1.846104	28830	28900	1510-1512	1.000000	65.4
trnC-GCA_p5	trnC-GCA	5	AGTTCAAATTTGCGACAGGT	TCAACGGCACAGAGTCAAAA	20	20	40.0	45.0	56.2	58.0	13.5	0.0	1.864787	28830	28900	1357-1359	1.000000	65.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GCGGTAGGAACTAGAATGAACA	CCTCGCTGGGATTGTAGTTC	22	20	45.5	55.0	57.9	58.1	0.0	0.0	2.178117	29917	30930	1148-1154	1.000000	63.1
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	GCGGTAGGAACTAGAATGAACA	AGTTCAATCGGTCAGAGCAC	22	20	45.5	50.0	57.9	57.9	0.0	0.0	2.205496	29917	30930	1133-1139	1.000000	62.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	GCGGTAGGAACTAGAATGAACA	TACACCTCGCTGGGATTGTA	22	20	45.5	50.0	57.9	58.1	0.0	10.4	2.264147	29917	30930	1152-1158	1.000000	62.4
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	20	20	45.0	55.0	55.8	58.1	0.0	0.0	2.287973	29917	30930	1136-1142	1.000000	62.3
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	20	20	45.0	50.0	55.8	57.9	0.0	0.0	2.315353	29917	30930	1121-1127	1.000000	62.0
psaA-ycf3_p1	psaA-ycf3	1	GTCTGGCCCATTCCTCAAAA	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.240462	42379	43055	795-795	1.000000	80.4
psaA-ycf3_p2	psaA-ycf3	2	TGGCCCATTCCTCAAAAGAC	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	58.1	57.8	0.0	0.0	0.240462	42379	43055	792-792	1.000000	80.4
psaA-ycf3_p3	psaA-ycf3	3	TGTTCTCGAGAAATGGCCTG	TTGAAGATCACGAGGCGTTT	20	20	50.0	45.0	57.9	57.8	28.6	0.0	0.262959	42379	43055	815-815	1.000000	80.2
psaA-ycf3_p4	psaA-ycf3	4	GTCTGGCCCATTCCTCAAAA	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.268234	42379	43055	799-799	1.000000	80.2
psaA-ycf3_p5	psaA-ycf3	5	TGGCCCATTCCTCAAAAGAC	TTGGTTGAAGATCACGAGGC	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.268234	42379	43055	796-796	1.000000	80.2
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	47145	48213	1130-1274	1.000000	78.2
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	15.5	10.2	0.488942	47145	48213	1162-1306	1.000000	77.3
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	47145	48213	1124-1268	1.000000	76.9
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	47145	48213	1162-1306	1.000000	76.1
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	CAGGTTTCCATACCAAGGCT	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.647198	47145	48213	1164-1308	1.000000	76.1
trnF-GAA-ndhJ_p1	trnF-GAA-ndhJ	1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180666	49049	49918	980-1003	1.000000	79.5
trnF-GAA-ndhJ_p2	trnF-GAA-ndhJ	2	AAAGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.261407	49049	49918	1023-1046	1.000000	78.8
trnF-GAA-ndhJ_p3	trnF-GAA-ndhJ	3	AAGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.261407	49049	49918	1022-1045	1.000000	78.8
trnF-GAA-ndhJ_p4	trnF-GAA-ndhJ	4	ACATAGATCAAGTGCTCCGC	TGAAAGTTGGATAGGCTGGC	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.359985	49049	49918	1060-1083	1.000000	78.0
trnF-GAA-ndhJ_p5	trnF-GAA-ndhJ	5	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	20	20	50.0	45.0	58.1	57.6	0.0	0.0	0.448485	49049	49918	1011-1034	1.000000	77.4
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	CCCTTCACTAATCGGTGGTG	AGAAGGTCTACGGTTCGAGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.221459	51610	52840	1369-1378	1.000000	79.6
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	ATCGGTGGTGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.306708	51610	52840	1359-1368	1.000000	78.9
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	GTGGTGCTAAAACTCCGGAA	AGAAGGTCTACGGTTCGAGT	20	20	50.0	50.0	58.1	57.8	5.2	0.0	0.310397	51610	52840	1355-1364	1.000000	78.9
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	CCCTTCACTAATCGGTGGTG	AGCCCAATGCAACAAAATGC	20	20	55.0	45.0	58.0	58.5	0.0	0.0	0.498235	51610	52840	1451-1460	1.000000	77.3
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	CCCTTCACTAATCGGTGGTG	GAAGGTCTACGGTTCGAGTC	20	20	55.0	55.0	58.0	57.5	0.0	0.0	0.565231	51610	52840	1368-1377	1.000000	76.8
atpB_p1	atpB	1	TCGATTAGGGGTCAGTACACA	ACTGATCAATTTGCTATCGGACA	21	23	47.6	39.1	57.9	58.2	0.0	11.8	4.320521	54355	55845	1601-1607	1.000000	46.0
atpB_p2	atpB	2	TCGATTAGGGGTCAGTACACA	CTGATCAATTTGCTATCGGACA	21	22	47.6	40.9	57.9	56.6	0.0	0.0	4.501811	54355	55845	1600-1606	1.000000	44.6
atpB_p3	atpB	3	TCGATTAGGGGTCAGTACACA	ACTGATCAATTTGCTATCGGAC	21	22	47.6	40.9	57.9	56.6	0.0	11.8	4.503913	54355	55845	1601-1607	1.000000	44.6
atpB_p4	atpB	4	TCGATTAGGGGTCAGTACACA	TGATCAATTTGCTATCGGACA	21	21	47.6	38.1	57.9	55.4	0.0	0.0	4.699665	54355	55845	1599-1605	1.000000	43.0
atpB_p5	atpB	5	TCGATTAGGGGTCAGTACACA	AACTGATCAATTTGCTATCGGAC	21	23	47.6	39.1	57.9	57.3	0.0	11.8	4.831598	54355	55845	1602-1608	1.000000	41.9
accD-psaI_p1	accD-psaI	1	TCAGCTCCACGGTTTCTTTC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	58.1	58.0	0.0	26.9	0.148050	60249	61141	993-1003	1.000000	80.3
accD-psaI_p2	accD-psaI	2	TCAGCTCCACGGTTTCTTTC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	58.1	57.9	0.0	26.8	0.224584	60249	61141	999-1009	1.000000	79.6
accD-psaI_p3	accD-psaI	3	TTCAGCTCCACGGTTTCTTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.368850	60249	61141	994-1004	1.000000	78.5
accD-psaI_p4	accD-psaI	4	TTTCAGCTCCACGGTTTCTT	CCATTGCAATTGCCGGAAAT	20	20	45.0	45.0	57.7	58.0	0.0	26.9	0.368850	60249	61141	995-1005	1.000000	78.5
accD-psaI_p5	accD-psaI	5	TTCAGCTCCACGGTTTCTTT	AAGAAGCCATTGCAATTGCC	20	20	45.0	45.0	57.7	57.9	0.0	26.8	0.445383	60249	61141	1000-1010	1.000000	77.9
ycf4-cemA_p1	ycf4-cemA	1	TTAGCCTATTTCTTGCGCGT	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	58.0	58.1	8.4	0.0	0.095186	62177	62912	831-855	1.000000	79.4
ycf4-cemA_p2	ycf4-cemA	2	AAAAGCTGCCGAATTAGCCT	GAGATCCACCAGGGCAAAAA	20	20	45.0	50.0	58.2	58.1	0.0	0.0	0.237221	62177	62912	844-868	1.000000	78.3
ycf4-cemA_p3	ycf4-cemA	3	TTAGCCTATTTCTTGCGCGT	GAGAGAGAGATCCACCAGGG	20	20	45.0	60.0	58.0	58.3	8.4	0.0	0.315131	62177	62912	837-861	1.000000	77.7
ycf4-cemA_p4	ycf4-cemA	4	CCTTGACTGGCACTGATGAG	GAGATCCACCAGGGCAAAAA	20	20	55.0	50.0	58.3	58.1	0.0	0.0	0.341258	62177	62912	890-914	1.000000	77.4
ycf4-cemA_p5	ycf4-cemA	5	TTAGCCTATTTCTTGCGCGT	TTCGGATTGCTGGGTATTCC	20	20	45.0	50.0	58.0	57.7	8.4	0.0	0.360388	62177	62912	895-919	1.000000	77.3
petA-psbJ_p1	petA-psbJ	1	CGTCCAAGGCCTTTTGTTCT	GGTTCATATTCCGGGTTGGG	20	20	50.0	55.0	58.7	58.3	17.6	0.0	0.993861	64818	66058	1368-1383	1.000000	74.3
petA-psbJ_p2	petA-psbJ	2	GATCCATTACGCGTCCAAGG	GGTTCATATTCCGGGTTGGG	20	20	55.0	55.0	58.8	58.3	18.6	0.0	1.094555	64818	66058	1379-1394	1.000000	73.4
petA-psbJ_p3	petA-psbJ	3	CGTCCAAGGCCTTTTGTTCT	CATATTCCGGGTTGGGTTCA	20	20	50.0	50.0	58.7	57.6	17.6	0.0	1.109968	64818	66058	1364-1379	1.000000	73.3
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	GTTCATATTCCGGGTTGGGT	20	20	50.0	50.0	58.7	57.6	17.6	0.0	1.111671	64818	66058	1367-1382	1.000000	73.3
petA-psbJ_p5	petA-psbJ	5	GATCCATTACGCGTCCAAGG	CATATTCCGGGTTGGGTTCA	20	20	55.0	50.0	58.8	57.6	18.6	0.0	1.210662	64818	66058	1375-1390	1.000000	72.5
rps8_p1	rps8	1	ATAACAGGAGGGGAGGATGG	GGGTACCGATAATTATAGTTCACC	20	24	55.0	41.7	57.9	56.5	0.0	5.6	5.648995	82542	82940	447-447	1.000000	40.2
rps8_p2	rps8	2	GGATAACAGGAGGGGAGGAT	GGGTACCGATAATTATAGTTCACC	20	24	55.0	41.7	57.6	56.5	0.0	5.6	5.949119	82542	82940	449-449	1.000000	40.2
rps8_p3	rps8	3	AGGATAACAGGAGGGGAGGA	GGGTACCGATAATTATAGTTCACC	20	24	55.0	41.7	58.7	56.5	0.0	5.6	6.194280	82542	82940	450-450	1.000000	40.2
rps8_p4	rps8	4	TAACAGGAGGGGAGGATGGA	GGGTACCGATAATTATAGTTCACC	20	24	55.0	41.7	59.0	56.5	0.0	5.6	6.500716	82542	82940	446-446	1.000000	40.2
rps8_p5	rps8	5	TAGGATAACAGGAGGGGAGG	GGGTACCGATAATTATAGTTCACC	20	24	55.0	41.7	56.9	56.5	0.0	5.6	6.620799	82542	82940	451-451	1.000000	40.2
rps8-rpl14_p1	rps8-rpl14	1	TGCTATTCGAACCGTTCCTT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	57.2	58.0	0.0	30.2	0.773663	82941	83192	419-420	1.000000	76.3
rps8-rpl14_p2	rps8-rpl14	2	TGCTATTCGAACCGTTCCTT	ACTCGAGTTTTTGGTGCGAT	20	20	45.0	45.0	57.2	58.1	0.0	13.6	0.875993	82941	83192	417-418	1.000000	75.5
rps8-rpl14_p3	rps8-rpl14	3	ATGCTATTCGAACCGTTCCT	GAACTCGAGTTTTTGGTGCG	20	20	45.0	50.0	57.0	58.0	0.0	30.2	1.000576	82941	83192	420-421	1.000000	74.5
rps8-rpl14_p4	rps8-rpl14	4	TGCTATTCGAACCGTTCCTTT	GAACTCGAGTTTTTGGTGCG	21	20	42.9	50.0	58.0	58.0	0.0	30.2	1.067054	82941	83192	419-420	1.000000	73.9
rps8-rpl14_p5	rps8-rpl14	5	TGCTATTCGAACCGTTCCTT	AACTCGAGTTTTTGGTGCGA	20	20	45.0	45.0	57.2	58.3	0.0	24.9	1.095953	82941	83192	418-419	1.000000	73.7
ndhF-rpl32_p1	ndhF-rpl32	1	AACACATTCCCACCAGTTCC	GTTCCCGATGGGTTAGCAAA	20	20	50.0	50.0	58.0	58.2	0.0	1.0	0.175645	115672	116109	1093-1113	1.000000	86.9
ndhF-rpl32_p2	ndhF-rpl32	2	CATTCCCACCAGTTCCCAAA	GTTCCCGATGGGTTAGCAAA	20	20	50.0	50.0	58.0	58.2	0.0	1.0	0.179263	115672	116109	1089-1109	1.000000	86.9
ndhF-rpl32_p3	ndhF-rpl32	3	AACACATTCCCACCAGTTCC	GCCCAGTGGAAACCGATTTA	20	20	50.0	50.0	58.0	58.2	0.0	12.0	0.175645	115672	116109	1034-1852	1.000000	83.6
ndhF-rpl32_p4	ndhF-rpl32	4	CATTCCCACCAGTTCCCAAA	GCCCAGTGGAAACCGATTTA	20	20	50.0	50.0	58.0	58.2	0.0	12.0	0.179263	115672	116109	1030-1848	1.000000	83.6
ndhF-rpl32_p5	ndhF-rpl32	5	GAAGAGGGAATTGTGCGGAT	GCCCAGTGGAAACCGATTTA	20	20	50.0	50.0	58.0	58.2	0.0	12.0	0.215144	115672	116109	1301-2119	1.000000	82.5
rpl32_p1	rpl32	1	AAAAGCCCACATACGACGAA	GTTCCCGATGGGTTAGCAAA	20	20	45.0	50.0	57.8	58.2	0.0	1.0	0.343872	116110	116271	747-767	1.000000	88.6
rpl32_p2	rpl32	2	AAAAGCCCACATACGACGAA	CAATCCCACCCATCAGTTCC	20	20	45.0	55.0	57.8	58.2	0.0	0.0	0.405092	116110	116271	762-782	1.000000	88.1
rpl32_p3	rpl32	3	AAAAGCCCACATACGACGAA	CCAATCCCACCCATCAGTTC	20	20	45.0	55.0	57.8	58.2	0.0	0.0	0.405092	116110	116271	763-783	1.000000	88.1
rpl32_p4	rpl32	4	AAAAGCCCACATACGACGAA	TTTCACCATGGCGGCTTATT	20	20	45.0	45.0	57.8	58.2	0.0	13.0	0.326845	116110	116271	1476-2159	1.000000	83.2
rpl32_p5	rpl32	5	AAAGCCCACATACGACGAAT	TTTCACCATGGCGGCTTATT	20	20	45.0	45.0	57.6	58.2	0.0	13.0	0.550583	116110	116271	1475-2158	1.000000	81.4