Hotspot

ndhD

SSC · 114869-116398 · 1530 bp

Back to Streptocarpus

Pi 0.00196

MarkerSeek score 37.60

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 9
pi: 0.00196
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ACCTCTTTTTGTCATTGTACA	AATATTTTGAGCACGGATTTT	33.3	28.6	53.3	52.3	1617 bp	1.0	40.4
ndhD_p2	ACCTCTTTTTGTCATTGTACA	ATATTTTGAGCACGGATTTTT	33.3	28.6	53.3	52.3	1616 bp	1.0	40.4
ndhD_p3	ACCTCTTTTTGTCATTGTACA	AATATTTTGAGCACGGATTTTT	33.3	27.3	53.3	53.3	1617 bp	1.0	40.4
ndhD_p4	ACCTCTTTTTGTCATTGTACAA	AATATTTTGAGCACGGATTTT	31.8	28.6	54.1	52.3	1617 bp	1.0	40.4
ndhD_p5	ACCTCTTTTTGTCATTGTACAA	ATATTTTGAGCACGGATTTTT	31.8	28.6	54.1	52.3	1616 bp	1.0	40.4