Hotspot

ndhA

SSC · 118928-121128 · 2201 bp

Back to Streptocarpus

Pi 0.00197

MarkerSeek score 61.00

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2201
variable_sites: 11
pi: 0.00197
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACTCAGTTAGCATAGGGAACA	CACCGGGTTTTATCAACTTGC	42.9	47.6	56.1	58.1	2368-2409 bp	1.0	49.8
ndhA_p2	GAACTCAGTTAGCATAGGGAACA	CACCGGGTTTTATCAACTTGC	43.5	47.6	57.9	58.1	2370-2411 bp	1.0	48.5
ndhA_p3	AACTCAGTTAGCATAGGGAACA	CACCGGGTTTTATCAACTTGC	40.9	47.6	56.8	58.1	2369-2410 bp	1.0	47.8
ndhA_p4	ACTCAGTTAGCATAGGGAACA	CCGGGTTTTATCAACTTGCA	42.9	45.0	56.1	56.6	2366-2407 bp	1.0	47.2
ndhA_p5	ACTCAGTTAGCATAGGGAACA	ACCGGGTTTTATCAACTTGC	42.9	45.0	56.1	56.6	2367-2408 bp	1.0	47.2