Hotspot

ccsA-ndhD

SSC · 114614-114868 · 255 bp

Back to Streptocarpus

Pi 0.0209

MarkerSeek score 58.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 255
variable_sites: 16
pi: 0.0209
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAATTCAGCAATTGTGGCGT	TCAGTCGAAAAGGTTGAAGT	45.0	40.0	57.9	54.9	416-421 bp	1.0	58.3
ccsA-ndhD_p2	CAATTCAGCAATTGTGGCGT	ACCCGTACTAGGTATTGGTATGT	45.0	43.5	57.9	58.2	462-467 bp	1.0	57.5
ccsA-ndhD_p3	TTCAGCAATTGTGGCGTCTA	TCAGTCGAAAAGGTTGAAGT	45.0	40.0	57.8	54.9	413-418 bp	1.0	57.4
ccsA-ndhD_p4	GCAATTGTGGCGTCTATTGG	TCAGTCGAAAAGGTTGAAGT	50.0	40.0	57.8	54.9	409-414 bp	1.0	57.3
ccsA-ndhD_p5	CAATTCAGCAATTGTGGCGT	TCCGGATTTTGTTCTTTCACT	45.0	38.1	57.9	55.7	438-443 bp	1.0	57.0