Hotspot

rpl32-trnL-UAG

SSC · 112608-113485 · 878 bp

Back to Streptocarpus

Pi 0.00349
MarkerSeek score 51.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
878
variable_sites
8
pi
0.00349
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGCAGCGTTAAAAGCGTTTT TTTAGGAGATGACATGCCGC 40.0 50.0 57.8 58.0 1077-1083 bp 1.0 78.7
rpl32-trnL-UAG_p2 TGCAGCGTTAAAAGCGTTTT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 57.8 57.9 1022-1028 bp 1.0 78.1
rpl32-trnL-UAG_p3 ATTGTGCAGCGTTAAAAGCG TTTAGGAGATGACATGCCGC 45.0 50.0 58.3 58.0 1081-1087 bp 1.0 77.8
rpl32-trnL-UAG_p4 TGCAGCGTTAAAAGCGTTTT AGCAGCGTGTCTACCAATTT 40.0 45.0 57.8 57.8 1015-1021 bp 1.0 77.7
rpl32-trnL-UAG_p5 TGCAGCGTTAAAAGCGTTTT CTAAGAGCAGCGTGTCTACC 40.0 55.0 57.8 57.8 1020-1026 bp 1.0 77.6