Hotspot

psbH-petB

LSC · 74859-75694 · 836 bp

Back to Streptocarpus

Pi 0.00159

MarkerSeek score 54.00

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 836
variable_sites: 4
pi: 0.00159
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	CTCCTTTGATGGGTCTTGCA	ATCGCCTGAATCTCAAGACG	50.0	50.0	57.8	57.8	1002-1009 bp	1.0	77.0
psbH-petB_p2	ACTCCTTTGATGGGTCTTGC	ATCGCCTGAATCTCAAGACG	50.0	50.0	57.8	57.8	1003-1010 bp	1.0	77.0
psbH-petB_p3	CTCCTTTGATGGGTCTTGCA	ATATCATCCGCAATCGCCTG	50.0	50.0	57.8	58.3	1014-1021 bp	1.0	76.6
psbH-petB_p4	ACTCCTTTGATGGGTCTTGC	ATATCATCCGCAATCGCCTG	50.0	50.0	57.8	58.3	1015-1022 bp	1.0	76.6
psbH-petB_p5	GGAACGACTCCTTTGATGGG	ATCGCCTGAATCTCAAGACG	55.0	50.0	58.3	57.8	1009-1016 bp	1.0	76.6