Hotspot

psaA-ycf3

LSC · 42060-42891 · 832 bp

Back to Streptocarpus

Pi 0.00409
MarkerSeek score 44.80
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
832
variable_sites
9
pi
0.00409
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 TGTGAGTTGGATCGCTTAGC ATCAAGCCGCTGAGTATTGG 50.0 50.0 58.0 58.0 1231-1252 bp 1.0 81.6
psaA-ycf3_p2 TGTGAGTTGGATCGCTTAGC GGAAACAGGCTATAGCGCTT 50.0 50.0 58.0 58.0 1213-1234 bp 1.0 81.6
psaA-ycf3_p3 TGTGAGTTGGATCGCTTAGC TGGTAACCGGACACCAAAAG 50.0 50.0 58.0 58.0 1380-1401 bp 1.0 81.5
psaA-ycf3_p4 GCATCAGCATGTAGGTTCCA TGGTAACCGGACACCAAAAG 50.0 50.0 58.0 58.0 1216-1237 bp 1.0 81.4
psaA-ycf3_p5 AGTTGGATCGCTTAGCCATG TGGTAACCGGACACCAAAAG 50.0 50.0 58.0 58.0 1376-1397 bp 1.0 81.3